A Comprehensive Guide to Western Blotting

Guide

Western blotting is a tried-and-true multistep process that scientists commonly use to detect and quantify proteins. A typical western blotting workflow involves sample preparation, electrophoresis, transfer, immunodetection, imaging, and analysis components, with a range of reagents and instruments available for scientists at each of these steps depending on their experimental goals.

In this guide, learn more about the options that scientists have at each step of a western blot protocol, how they impact results and data, and discover the right choices for a given experiment.